Replication forks are arrested at specific sequences to facilitate a variety of DNA transactions. Forks also stall at sites of DNA damage, and the regression of stalled forks without rescue can cause ...

DNA synthesis is an accurate and very processive phenomenon; nevertheless, replication fork progression on chromosomes can be impeded by DNA lesions, DNA secondary structures, or DNA-bound proteins.

Mouse ES cells present active origin firing and replication forks that progress slowly throughout S phase. These features are integral aspects of genome replication ensuring genome integrity in ES ...

Our lab studies the mechanism of eukaryotic chromosome replication. Chromosomes are the carriers of the genetic and epigenetic information and faithful chromosome replication is of fundamental ...

Embryonic stem (ES) cells are pluripotent stem cells that can produce all cell types of an organism. ES cells proliferate rapidly and have been thought to experience high levels of intrinsic ...

When an SSB appears, polymerase epsilon approaches the nick before retracing its steps thanks to its exonuclease, allowing fork reversal to take place. The nick is repaired as an SSB, and replication ...

The human genome consists of 3 billion base pairs, and when a cell divides, it takes about seven hours to complete making a copy of its DNA. That's almost 120,000 base pairs per second. At that ...

Despite the importance of DNA replication, numerous aspects of this process are still poorly understood. One fundamental question is: how do replication forks efficiently progress through chromatin?

ETX-19477 is a potent, selective inhibitor of poly (ADP-ribose) glycohydrolase (PARG), an enzyme that plays a role in the DNA ...